Multiparametric Color Tendency Analysis (MCTA): A Method to Analyze Several Flow Cytometry Labelings Simultaneously

Multiparametric analysis of apoptosis by flow and image cytometry.

Flow cytometric assays for apoptosis are now in widespread use. The multiparametric nature of flow cytometry allows multiple assays for several apoptotic characteristics to be combined in a single sample, providing a powerful tool for elucidating the complex progression of apoptotic death in a variety of cell types. This chapter describes one such assay, allowing simultaneous analysis of caspas...

Multiparametric Analysis of Aquatic Organisms Using Flow Cytometry

Environmental research on aquatic microorganisms, such as phytoplankton, aquatic bacteria, and aquatic viruses, often takes advantage of their natural chlorophyll, phycobilins, and other intrinsic fluorescent pigments. Although epifluorescence microscopy is still used frequently, flow cytometry has become a key research tool due to its ability to provide multiparametric analysis at the single-c...

New Method to Disaggregate and Analyze Single Isolated Helminthes Cells Using Flow Cytometry: Proof of Concept

In parasitology, particularly in helminthes studies, several methods have been used to look for the expression of specific molecules, such as RT-PCR, western blot, 2D-electrophoresis, and microscopy, among others. However, these methods require homogenization of the whole helminth parasite, preventing evaluation of individual cells or specific cell types in a given parasite tissue or organ. Als...

Diagnostic Application of Two-Color Flow Cytometry

Recent immunophenotypic studies of hairy cell leukemia (HCL) have suggested specific patterns of immunoreactivity that may aid in diagnosis. We studied peripheral blood (PB) from 1 61 cases of HCL using two-color direct immunofluorescence flow cytometry and an extended panel of antibody combinations. Circulating hairy cells were identified by immunophenotypic features in 92% of the cases and co...

Studying Mouse Thymocyte Development using Multiparametric Flow Cytometry: An Efficient Method to Improve an 8-Color Panel on the BD FACSVerseTM System Application Note